Some properties of the bacitracin polypeptides.
نویسندگان
چکیده
Newton & Abraham (1950) found that crude bacitracin could be resolved into a series of similar polypeptides by counter-current distribution between solvents. Better resolution and larger amounts ofthe products have now been obtained by using a greater number of transfers and starting with more concentrated solutions than were used in the earlier experiments. It has been possible, by a single distribution, to prepare the various bacitracins in quantities sufficient for some of their chemical and biological properties to be compared. Hausmann, Ahrens & Harfenist (1951) and containing 101 tubes. The solvent system consisted of n-butanol (4 vol.), amyl alcohol (1 vol.), in equilibrium with 0-05M-potassium phosphate buffer (5 vol.), at pH 7-0 (Newton & Abraham, 1950). Analysis of the distribution curve (see Experimental) shows that the crude bacitracin was resolved, or partly resolved, into at least ten different components. Table 1 shows the proportions in which nine of these components were present, their partition coefficients (K) in the solvent system used, and
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 53 4 شماره
صفحات -
تاریخ انتشار 1953